The 2015 Gordon Conference on Molecular Membrane Biology will feature cutting-edge research on organelle biology and membrane traffic, with fundamental implications across many areas of human health and development. Invited talks will cover a wide range of topics including membrane protein insertion and translocation; mechanisms and regulation of membrane shaping, fission, and fusion; the molecular basis of organellar identity and interactions; lipid biology; and metabolism and autophagy. In addition, two sessions will be devoted to new frontiers in molecular membrane biology. A longstanding strength of this Gordon Conference has been the multidisciplinary nature of the research reported, drawing on methodological advances in areas ranging from high-throughput genetics and proteomics to structural biology and super-resolution microscopy. Attendance by under-represented minorities, students, postdoctoral fellows, junior faculty, and faculty from primarily undergraduate institutions is encouraged.